| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fried, Riley L (8)
|
MRLIAC |
42.63 |
41.99 |
-0.64 |
| 2 |
Herr, Emily A (6)
|
MRTVSC |
56.77 |
43.20 |
-13.57 |
| 3 |
Seale, Sarah E (8)
|
MRTVSC |
59.16 |
44.93 |
-14.23 |
| 4 |
Dimaculangan, Annabel (7)
|
MRTVSC |
1:00.23 |
45.46 |
-14.77 |
| 5 |
McKenna, Mary K (8)
|
MRBBSC |
47.93 |
45.50 |
-2.43 |
| 6 |
Eng, Amelie R (8)
|
MRBBSC |
43.71 |
45.88 |
2.17 |
| 7 |
Tan, Sayjel E (8)
|
MRBBSC |
44.20 |
46.30 |
2.10 |
| 8 |
Karras, Nicoletta E (8)
|
MRLIAC |
43.00 |
47.29 |
4.29 |
| 9 |
Bances, Alayna H (7)
|
MRTVSC |
49.13 |
50.32 |
1.19 |
| 10 |
Zorn, Shannon G (8)
|
MRLIAC |
49.00 |
50.60 |
1.60 |
| 11 |
Gold, Carly P (8)
|
MRLIAC |
1:00.00 |
54.74 |
-5.26 |
| 12 |
Demirci, Morgan (8)
|
MRHAA |
57.58 |
57.43 |
-0.15 |
| 13 |
Johannesen, Charlotte (8)
|
MRBBSC |
58.55 |
57.50 |
-1.05 |
| 14 |
Sforza, Sophia (6)
|
MRBBSC |
1:00.55 |
1:03.24 |
2.69 |
| 15 |
Zhu, Grace (8)
|
MRBBSC |
57.66 |
1:04.58 |
6.92 |